World Library  

Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Overexpression of Atdreb1A Causes a Severe Dwarf Phenotype by Decreasing Endogenous Gibberellin Levels in Soybean Glycine Max L. Merr., Volume 7

By Blazquez, Miguel A.

Click here to view

Book Id: WPLBN0003957910
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Overexpression of Atdreb1A Causes a Severe Dwarf Phenotype by Decreasing Endogenous Gibberellin Levels in Soybean Glycine Max L. Merr., Volume 7  
Author: Blazquez, Miguel A.
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Blazquez, M. A. (n.d.). Plos One : Overexpression of Atdreb1A Causes a Severe Dwarf Phenotype by Decreasing Endogenous Gibberellin Levels in Soybean Glycine Max L. Merr., Volume 7. Retrieved from http://worldjournal.org/


Description
Description : Gibberellic acids (GAs) are plant hormones that play fundamental roles in plant growth and developmental processes. Previous studies have demonstrated that three key enzymes of GA20ox, GA3ox, and GA2ox are involved in GA biosynthesis. In this study, the Arabidopsis DREB1A gene driven by the CaMV 35S promoter was introduced into soybean plants by Agrobacterium- mediated transformation. The results showed that the transgenic soybean plants exhibited a typical phenotype of GA-deficient mutants, such as severe dwarfism, small and dark-green leaves, and late flowering compared to those of the non-transgenic plants. The dwarfism phenotype was rescued by the application of exogenous GA3 once a week for three weeks with the concentrations of 144 mM or three times in one week with the concentrations of 60 mM. Quantitative RT-PCR analysis revealed that the transcription levels of the GA synthase genes were higher in the transgenic soybean plants than those in controls, whereas GA-deactivated genes except GmGA2ox4 showed lower levels of expression. The transcript level of GmGA2ox4 encoding the only deactivation enzyme using C20-GAs as the substrates in soybean was dramatically enhanced in transgenic plants compared to that of wide type. Furthermore, the contents of endogenous bioactive GAs were significantly decreased in transgenic plants than those of wide type. The results suggested that AtDREB1A could cause dwarfism mediated by GA biosynthesis pathway in soybean.

 
 


Copyright © World Library Foundation. All rights reserved. eBooks from World Journals, Database of Academic Research Journals are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.